Twitterers don't like to be sold... they want to communicate, or learn or ???
We've just started using it on a LiveSEO project that tweets and blogs about a real time
SEO project on one of our ecommerce sites.
find something unique and genuinely useful - and setup a tasteful autoresponder like TweetLater or SocialToo that will let them know that you'd like to have genuine dialogue with them about your industry - but don't sell them in the autoresponder.
There are several e-books on Twitter tips, I'd recommend reading them
I guess I could just sum everything up and say - "Be Genuine"
Relationships take time to develop, and Twitter is a relationship building tool, not an instant checkout line.